We will be using the enzyme catecholase or catechol oxidase that is common in plants; it is present in the freshly squeezed potato juice that has been provided. Did you support or refute your hypothesis? This substrate will fill the active sites on the… 2115 Words 9 Pages the concentration of sucrose found in the potato, then the potato would decrease in mass. Exact amounts do not matter, as long as they are relatively close. After 5 minutes add 10 drops 1% catechol. Begin with about 40 grams of peeled potato and blend it in about 100ml of water and ice. This emphasizes that the liver and potato are not part of the reaction. We conducted the experiment so that we were able to have a control group utilizing water while we were able to change the variables with hydrogen peroxide, ammonia, and vinegar.
It is usually easy to identify the names of enzymes because they end in -ase. Since enzyme is made up of protein which easily affected by varies temperature. Acid, Base, Catalase 1054 Words 5 Pages conditions in potato juice extract Introduction The presence of quinones prevents infection of microorganisms because it is toxic to them Danyk, H, 2013. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? We are all born with this enzyme. The answer to the question was proved to be that more concentration of amylase speeds up the reaction time. The ammonia however does not react with the catalase of the potato as the hydrogen peroxide did however the ammonia did have a reaction with the potato after it was removed from the solution. Enzymes are produced by living cells and they speed up reactions because they are biological catalysts.
You can observe what happens to the function of the enzyme when its cofactor is removed. If the potato were to be boiled or frozen, then the catalase would be damaged and would not cause a reaction as it had done with the raw potato. Health and safety checked, September 2008 Downloads Download the student sheet with questions and answers Web links Microscale investigations with catalase — which has been transcribed onto this site at Investigating catalase activity in different plant tissues. Base, Buffer solution, Catalysis 1290 Words 6 Pages. Materials: Potato Test tube or other small container Hydrogen Peroxide 1. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity.
Obtain about 1g ground potato. Enzymes are molecules that enable the chemical reactions that occur in all living things on earth. Catechol is found beneath the skin of many plants such. It is unstable which makes it very reactive. They function by reducing the activation energy, or energy required to start a reaction. Also, the reaction is so vigorous that bubbles of mixture can carry pieces of liver into the delivery tube.
After testing liver, apple, and potato it was concluded that liver contained the most catalase. Observations: Observations Rate of Reaction Interpretations A Sand - Sand piled up at the bottom of 0 - There is no reaction between sand and. Hypothesis: If the peroxidase enzyme is reacting with potatoes at temperatures extremely high, the reaction will become denatured causing no reaction to occur. The enzyme studied in this lab was catalase. Target response: 1 and 2. In the second part of the experiment, the intensity of the reaction in ascending order was apple, potato, and liver.
An enzyme is a protein and is a catalyst to chemical reactions. The organism which contained the cells is gone but the cells are still present and active in certain conditions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. You will perform the experiment, write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. What did grinding the potato up do to the rate? Class practical or demonstration Hydrogen peroxide H 2O 2 is a by-product of respiration and is made in all living cells. Catalase is such an anti-oxidant molecule.
Enzymes are proteins made up of long chains of amino acids. Use distilled water where H 2O is indicated. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. Add several drops of H2O2 to the potato blend in the bottom of the test tube. Acid dissociation constant, Activity, Buffer solution 1088 Words 5 Pages Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. Although our hypothesis was primarily correct in that hydrogen peroxide would have the longest reaction time, we did not expect for ammonia and vinegar to have absolutely no reaction with the catalase at all. Results When the substrate of hydrogen peroxide was added to the substances each performed a vastly different reaction.
The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. H2O2 is toxic to our bodies and catalase or peroxidase breaks down the H2O2 into H2O and O2, which our bodies are able to use. Each enzyme has only one substrate that it breaks down. At low temperatures molecules move very slowly, and the chance of their colliding and reacting with one another is very low. To test our question, we varied a combination of substrate and buffer, totaling 6mL, with a constant amount of 2 drops of catalyst. In the third experiment, it was inferred that as the acidity increased in the substrate, the rate of reaction decreased.
I think that grinding the materials will increase enzyme activity. The potato because there are few enzymes and it continues until the break down of the H2O2 is complete. Record the exact mass and add it to test tube 3. Both low pH levels and high pH levels produced a low amount of catalase activity. This is called the fruit browning reaction.