These plants eventually grow to be a brand new plant that is genetically identical to the parent plant it came from. One bacterium was gram positive and one bacterium was gram negative. The writing should be simple and easy to understand. After viewing it under a light microscope, pink rods were observed, confirming this. A lot of the air we breathe….
After the Gram stain was determined, specific biochemical tests were performed. First we made sure that all the equipment was ready in front of us and all. Microbe B was a gram-negative… Discussion: Each test is a pertinent contribution to drawing an educated conclusion. Bacteria, Bacteriology, Enterobacteriaceae 788 Words 3 Pages 31 October 2011 Identification and observations of Citrobacter Freundii Introduction Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. Listen Read phonetically Dictionary - View detailed dictionary 1. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. The number one thing is to wash your hands.
Simmons citrate agar utilizes sodium citrate as its only carbon source and ammonium phosphate as the nitrogen source. San Francisco: Pearson Benjamin Cummings, 2010 3. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Although only a few micrometers in length, bacteria can still be examined through the use of a simple light microscope. The results from the Gram Stain of unknown number 24 showed that unknown number 24 has bacillus shaped cell morphology, holding a dark-pink color confirming it to be a gram negative organism indeed.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology. To figure out the characteristics, T-streak was performed to isolate… Determining an Unknown Through Deferential Stains and Biochemical Tests Introduction There are many reasons for knowing the identity of microorganisms. Materials and Methods A test tube with an unknown microorganism will be retrieved. The urea hydrolysis test determines which organisms are able or unable to hydrolyze urea with the enzyme urease. Saint Louis Community College at Meramec, 2011. The absence of fermentation, the entire slant will be red which means that peptone was catabolized both aerobically and anaerobically which produced products that are alkaline.
After conducting each of these tests, the unknown bacteria number 24 was concluded to be Proteus mirabilis. Urea hydrolysis to ammonia by urease-positive organisms will overcome the buffer in the medium and change it from orange to pink Leboffe and Pierce 187. Even with that stated I have grown to enjoy this experiment and have learned so much valuable information that will benefit me in my nursing career. To confirm the Gram-negative properties of the bacteria a Gram Stain procedure was carried out and to distinguish our given unknown from the remaining… Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. The T- streak isolation method will make sure there is no contamination in the unknown.
The Simmons citrate test was performed, no color change meaning negative result. This aspect was crucial for many reasons; within the medical field, knowing what bacterium a patient has been infected with could mean the difference between life and death. The purpose of this test was to determine if the bacterium could ferment mannitol McDonald et al. After performing the Gram stain to determine that the unknown was a Gram negative rod, the organism was grown on slant for use in inoculating the rest of the biochemical tests. Bacteria are necessary for the maintenance of human health. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines. The test was performed only on the gram-positive culture.
Table 1 lists all of the biochemical tests, their purpose and results. While Gram staining shows the difference between Gram Positive… The objective of this lab was to isolate and identify an unknown Gram positive and Gram negative bacteria through the use of various microbiological techniques. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Like the Maltose, this test utilized the same procedures, loop inoculating and allowing for an incubation period. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. All of the previous results were than compared to gram negative unknown chart and the only bacteria that had the same results was Escherichia coli.
The organisms showed weak motility, proving that the unknown organism was flagellated and can produce movement. With Escherichia coli being the only bacteria that had a negative result for this test, for the remaining of the lab report the unknown will be simply referred to as E. The gram stain turned out to be a positive rod gram stain. The Voges-Proskauer test came back with a negative result. This organism is gram- positive, single celled, arranged in grape-like clusters, and cocci in shape Bukhari, 2004.
The gram stain is a differential stain that utilizes a primary stain of crystal violet i. Lab Manual for General Mircobiology. It had many well isolated colonies. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. Knowing what organism will give the student an idea of the genus and species that can cause diseases, how it can grow and reproduce including how this type of bacteria can be treated and killed, what type of antibiotic it will be susceptible to. Bacteria, Bacteriology, Escherichia coli 454 Words 2 Pages Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium.
Originally, a sample from the Unknown tube was supposed to be inoculated on a nutrient agar plate using the quadrant streak technique. The casein test was used to show which organism would produce the enzyme casease, which hydrolyzes the milk protein casein. The first microorganism analyzed was Staphylococcus epidermidis. The citrate test was performed by streaking a Simmons Citrate slant with an inoculating loop. There are five different isotypes known in mammals to perform different roles and to direct a specific immune response for the antigen encountered.